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These cells were grown in RPMI 1640 media provided with 10% fetal bovine serum, 1% penicillin, and 1% streptomycin, and then incubated with 5% CO 2 at 37C

Some scholars have used the synoviocyte mitochondrial gene encoding NADH dehydrogenase 1 as an entry point in order to study the mutation level of synoviocyte mtDNA in rheumatoid arthritis, and have found that the number of mutations in RA synoviocyte mtDNA is approximately twice as high as that of the OA group, and that RA fibroblasts are expressed at a mutation load of 2.3 amino acid changes per thousand base pairs, whereas in tissues, there are 2.3 changes per 2.5 amino acid changes per thousand base pairs

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